Comparison of Plasmodium falciparum allelic frequency distribution in different endemic settings by high-resolution genotyping.

ABSTRACT:

Background

The diversity of genotyping markers of Plasmodium falciparum depends on transmission intensity. It has been reported that the diversity of the merozoite surface protein 2 (msp2) is greater in areas of high compared to low endemicity, however, results for msp1 were inconsistent. These previous reports relied on low resolution genotyping techniques.

Methods

In the present study, a high-resolution capillary electrophoresis-based technique was applied to genotype samples from areas of different endemicity in Papua New Guinea and Tanzania. For both endemic settings, the diversity of msp1 and msp2 was investigated; the mean multiplicity of infection (MOI) and the FST values were determined to investigate whether more accurate sizing generates different results.

Resul

SUBMITTER: Schoepflin S 

PROVIDER: S-EPMC2774868 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature