Large-scale profiling of protein palmitoylation in mammalian cells.

ABSTRACT: S-palmitoylation is a pervasive post-translational modification required for the trafficking, compartmentalization and membrane tethering of many proteins. We demonstrate that the commercially available compound 17-octadecynoic acid (17-ODYA) can serve as a bioorthogonal, click chemistry probe for in situ labeling, identification and verification of palmitoylated proteins in human cells. We identified approximately 125 predicted palmitoylated proteins, including G proteins, receptors and a family of uncharacterized hydrolases whose plasma membrane localization depends on palmitoylation.

SUBMITTER: Martin BR 

PROVIDER: S-EPMC2775068 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature