Highly error-free role of DNA polymerase eta in the replicative bypass of UV-induced pyrimidine dimers in mouse and human cells.

ABSTRACT: Cyclobutane pyrimidine dimers (CPDs) constitute the most frequent UV-induced DNA photoproduct. However, it has remained unclear how human and other mammalian cells mitigate the mutagenic and carcinogenic potential of CPDs emanating from their replicative bypass. Here, we examine in human cells the roles of translesion synthesis (TLS) DNA polymerases (Pols) in the replicative bypass of a cis-syn TT dimer carried on the leading or the lagging strand DNA template in a plasmid system we have designed, and we determine in mouse cells the frequencies and mutational spectra generated from TLS occurring specifically opposite CPDs formed at TT, TC, and CC dipyrimidine sites. From these studies we draw the following conclusions: (i) TLS makes a very prominent contribution to CPD bypass on both the D

SUBMITTER: Yoon JH 

PROVIDER: S-EPMC2775276 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature