Site-specific recombination for the modification of transgenic strains of the Mediterranean fruit fly Ceratitis capitata.

ABSTRACT: Insect transgenesis is mainly based on the random genomic integration of DNA fragments embedded into non-autonomous transposable elements. Once a random insertion into a specific location of the genome has been identified as particularly useful with respect to transgene expression, the ability to make the insertion homozygous, and lack of fitness costs, it may be advantageous to use that location for further modification. Here we describe an efficient method for the modification of previously inserted transgenes by the use of the site-specific integration system from phage phiC31 in a tephritid pest species, the Mediterranean fruit fly Ceratitis capitata. First, suitable transgenic strains with randomly integrated attP landing sites within transposon-based vectors were identified by molecu

SUBMITTER: Schetelig MF 

PROVIDER: S-EPMC2775304 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature