A hierarchical MS2/MS3 database search algorithm for automated analysis of phosphopeptide tandem mass spectra.

ABSTRACT: A novel hierarchical MS(2)/MS(3) database search algorithm has been developed to analyze MS(2)/MS(3) phosphopeptides proteomic data. The algorithm is incorporated in an automated database search program, MassMatrix. The algorithm matches experimental MS(2) spectra against a supplied protein database to determine candidate peptide matches. It then matches the corresponding experimental MS(3) spectra against those candidate peptide matches. The MS(2) and MS(3) spectra are used in concert to arrive at peptide matches with overall higher confidence rather than combining MS(2) and MS(3) data searched separately. Receiver operating characteristic analysis showed that hierarchical MS(2)/MS(3) database searches with MassMatrix had better sensitivity and specificity than the two-stage MS(2)/MS(3) d

SUBMITTER: Xu H 

PROVIDER: S-EPMC2775432 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature