Visualisation and quantitative analysis of the rodent malaria liver stage by real time imaging.

ABSTRACT: The quantitative analysis of Plasmodium development in the liver in laboratory animals in cultured cells is hampered by low parasite infection rates and the complicated methods required to monitor intracellular development. As a consequence, this important phase of the parasite's life cycle has been poorly studied compared to blood stages, for example in screening anti-malarial drugs. Here we report the use of a transgenic P. berghei parasite, PbGFP-Luc(con), expressing the bioluminescent reporter protein luciferase to visualize and quantify parasite development in liver cells both in culture and in live mice using real-time luminescence imaging. The reporter-parasite based quantification in cultured hepatocytes by real-time imaging or using a microplate reader correlates very well with es

SUBMITTER: Ploemen IH 

PROVIDER: S-EPMC2775639 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature