Whole-genome analysis of pseudorabies virus gene expression by real-time quantitative RT-PCR assay.

ABSTRACT:

Background

Pseudorabies virus (PRV), a neurotropic herpesvirus of pigs, serves as an excellent model system with which to investigate the herpesvirus life cycle both in cultured cells and in vivo. Real-time RT-PCR is a very sensitive, accurate and reproducible technique that can be used to detect very small amounts of RNA molecules, and it can therefore be applied for analysis of the expression of herpesvirus genes from the very early period of infection.

Results

In this study, we have developed and applied a quantitative reverse transcriptase-based real-time PCR technique in order to profile transcription from the whole genome of PRV after lytic infection in porcine kidney cells. We calculated the relative expression ratios in a novel way, which allowed us to compare differ

SUBMITTER: Tombacz D 

PROVIDER: S-EPMC2775753 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature