Hepatic gene expression profiling reveals protective responses in Atlantic salmon vaccinated against furunculosis.

ABSTRACT:

Background

Furunculosis, a disease caused with gram negative bacteria Aeromonas salmonicida produces heavy losses in aquaculture. Vaccination against furunculosis reduces mortality of Atlantic salmon but fails to eradicate infection. Factors that determine high individual variation of vaccination efficiency remain unknown. We used gene expression analyses to search for the correlates of vaccine protection against furunculosis in Atlantic salmon.

Results

Naïve and vaccinated fish were challenged by co-habitance. Fish with symptoms of furunculosis at the onset of mass mortality (LR - low resistance) and survivors (HR - high resistance) were sampled. Hepatic gene expression was analyzed with microarray (SFA2.0 - immunochip) and real-time qPCR. Comparison of LR and HR indicated

SUBMITTER: Skugor S 

PROVIDER: S-EPMC2775754 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature