Genome-wide analysis of immune activation in human T and B cells reveals distinct classes of alternatively spliced genes.

ABSTRACT: Alternative splicing of pre-mRNA is a mechanism that increases the protein diversity of a single gene by differential exon inclusion/exclusion during post-transcriptional processing. While alternative splicing is established to occur during lymphocyte activation, little is known about the role it plays during the immune response. Our study is among the first reports of a systematic genome-wide analysis of activated human T and B lymphocytes using whole exon DNA microarrays integrating alternative splicing and differential gene expression. Purified human CD2(+) T or CD19(+) B cells were activated using protocols to model the early events in post-transplant allograft immunity and sampled as a function of time during the process of immune activation. Here we show that 3 distinct classes of al

SUBMITTER: Grigoryev YA 

PROVIDER: S-EPMC2775942 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature