Modulation of NFAT-5, an outlying member of the NFAT family, in human keratinocytes and skin.

ABSTRACT:

Background

Cyclosporin A (CsA) and tacrolimus block T cell activation by inhibiting the phosphatase calcineurin and preventing translocation from the cytoplasm to the nucleus of the transcription factor Nuclear Factor of Activated T cells (NFAT). NFAT compose a family of transcription factors that are turned on during T cell activation.

Aims

To study the expression of NFAT-5 mRNA and protein in normal human keratinocytes and to investigate the cellular and subcellular pattern of expression of NFAT-5 in normal human skin and psoriasis, and analyze effects of different agonists and ultraviolet radiation on NFAT-5 in normal human skin.

Methods

Tissue cultures, Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR), Western analysis, immunostaining, confocal microscopy.

SUBMITTER: Al-Daraji WI 

PROVIDER: S-EPMC2776318 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature