ERalpha increases expression and interacts with TERT in cataractous canine lens epithelial cells.

ABSTRACT:

Purpose

Estrogen receptor alpha (ERalpha) expression has previously been evaluated in lens epithelial cells (LEC). However, its function in the lens has not been determined. One potential function may be its interaction with the catalytic subunit of telomerase (TERT), which is present in normal LEC and higher in LEC that have undergone epithelial to mesenchymal transition (EMT). ERalpha is known to play a role in EMT, a process that may also involve TERT.

Methods

A commercially available transcription factor array was used to evaluate potential interactions between TERT and other proteins in normal and cataractous LEC. Based on these findings, ERalpha protein and mRNA expressions were measured using western blot analysis, immunohistochemical staining, and quantitative revers

SUBMITTER: Colitz CM 

PROVIDER: S-EPMC2776345 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature