Phylogenetic analysis of Newcastle disease viruses isolated from waterfowl in the upper midwest region of the United States.

ABSTRACT:

Background

This study was conducted to characterize Newcastle disease virus (NDV) isolates obtained from waterfowl from the Upper Midwest region of the United States. A total of 43 NDVs were isolated by inoculation of cloacal samples in embryonated chicken eggs. These isolates were obtained from 24 mallards, seven American green-winged teals, six northern pintails, four blue-winged teals, and two wood ducks. Partial sequences of fusion gene were analyzed to determine the pathotypes and genotypes involved.

Results

Deduced amino acid sequence of the cleavage site of fusion (F) protein revealed that all isolates had avirulent motifs. Of the 43 isolates, 23 exhibited sequence motif of (111)GGKQGRL(117) at the cleavage site, 19 exhibited (111)GEKQGRL(117) while one isolate showed

SUBMITTER: Jindal N 

PROVIDER: S-EPMC2776597 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature