Expression profiling of cardiac genes in human hypertrophic cardiomyopathy: insight into the pathogenesis of phenotypes.

ABSTRACT:

Objectives

The goal of this study was to identify genes upregulated in the heart in human patients with hypertrophic cardiomyopathy (HCM).

Background

Hypertrophic cardiomyopathy is a genetic disease caused by mutations in contractile sarcomeric proteins. The molecular basis of diverse clinical and pathologic phenotypes in HCM remains unknown.

Methods

We performed polymerase chain reaction-select complementary DNA subtraction between normal hearts and hearts with HCM and screened subtracted libraries by Southern blotting. We sequenced the differentially expressed clones and performed Northern blotting to detect increased expression levels.

Results

We screened 288 independent clones, and 76 clones had less than twofold increase in the signal intensity and were co

SUBMITTER: Lim DS 

PROVIDER: S-EPMC2776821 | biostudies-literature | 2001 Oct

REPOSITORIES: biostudies-literature