Alterations of BRAF and HIPK2 loci predominate in sporadic pilocytic astrocytoma.

ABSTRACT:

Objective

Independent studies have previously demonstrated that both the HIPK2 and BRAF genes are amplified and rearranged, respectively, in pilocytic astrocytomas (PAs). The purpose of this study was to further investigate the frequency of BRAF and HIPK2 alterations in PAs, the concordance of these events, and their relationship to clinical phenotype.

Methods

We performed extensive characterization by array-based copy number assessment (aCGH), HIPK2 copy number analysis, and BRAF rearrangement and mutation analysis in a set of 79 PAs, including 9 tumors from patients with neurofibromatosis type 1 (NF1).

Results

We identified 1 of 3 previously identified BRAF rearrangements in 42/70 sporadic PAs. An additional 2 tumors with no rearrangement also exhibited BRAF mutatio

SUBMITTER: Yu J 

PROVIDER: S-EPMC2777068 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature