Topological analysis of a haloacid permease of a Burkholderia sp. bacterium with a PhoA-LacZ reporter.

ABSTRACT:

Background

2-Haloacids can be found in the natural environment as degradative products of natural and synthetic halogenated compounds. They can also be generated by disinfection of water and have been shown to be mutagenic and to inhibit glyceraldehyde-3-phosphate dehydrogenase activity. We have recently identified a novel haloacid permease Deh4p from a bromoacetate-degrading bacterium Burkholderia sp. MBA4. Comparative analyses suggested that Deh4p is a member of the Major Facilitator Superfamily (MFS), which includes thousands of membrane transporter proteins. Members of the MFS usually possess twelve putative transmembrane segments (TMS). Deh4p was predicted to have twelve TMS. In this study we characterized the topology of Deh4p with a PhoA-LacZ dual reporters system.

Result

SUBMITTER: Tse YM 

PROVIDER: S-EPMC2777183 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature