Dosage regulation of the active X chromosome in human triploid cells.

ABSTRACT: In mammals, dosage compensation is achieved by doubling expression of X-linked genes in both sexes, together with X inactivation in females. Up-regulation of the active X chromosome may be controlled by DNA sequence-based and/or epigenetic mechanisms that double the X output potentially in response to autosomal factor(s). To determine whether X expression is adjusted depending on ploidy, we used expression arrays to compare X-linked and autosomal gene expression in human triploid cells. While the average X:autosome expression ratio was about 1 in normal diploid cells, this ratio was lower (0.81-0.84) in triploid cells with one active X and higher (1.32-1.4) in triploid cells with two active X's. Thus, overall X-linked gene expression in triploid cells does not strictly respond to an autoso

SUBMITTER: Deng X 

PROVIDER: S-EPMC2777382 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature