Genome-wide silencing in Drosophila captures conserved apoptotic effectors.

ABSTRACT: Apoptosis is a conserved form of programmed cell death firmly established in the aetiology, pathogenesis and treatment of many human diseases. Central to the core machinery of apoptosis are the caspases and their proximal regulators. Current models for caspase control involve a balance of opposing elements, with variable contributions from positive and negative regulators among different cell types and species. To advance a comprehensive view of components that support caspase-dependent cell death, we conducted a genome-wide silencing screen in the Drosophila model. Our strategy used a library of double-stranded RNAs together with a chemical antagonist of Inhibitor of apoptosis proteins (IAPs) that simulates the action of native regulators in the Reaper and Smac (also known as Diablo) fami

SUBMITTER: Chew SK 

PROVIDER: S-EPMC2777527 | biostudies-literature | 2009 Jul

REPOSITORIES: biostudies-literature