Resonance Energy Transfer Between Luminescent Quantum Dots and Diverse Fluorescent Protein Acceptors.

ABSTRACT: We characterized the resonance energy transfer interactions for conjugates consisting of QD donors self-assembled with three distinct fluorescent protein acceptors: two monomeric fluorescent proteins, the dsRed derivative mCherry or yellow fluorescent protein and the multi-chromophore b-phycoerythrin light harvesting complex. Using steady-state and time-resolved fluorescence, we showed that nonradiative transfer of excitation energy in these conjugates can be described within the Förster dipole-dipole formalism, with transfer efficiencies that vary with the degree of spectral overlap, donor-acceptor separation distance and the number of acceptors per QD. Comparison between the quenching data and simulation of the conjugate structures indicated that while energy transfer to monomeric protei

SUBMITTER: Medintz IL 

PROVIDER: S-EPMC2777665 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature