Small molecule microarrays of RNA-focused peptoids help identify inhibitors of a pathogenic group I intron.

ABSTRACT: Peptoids that inhibit the group I intron RNA from Candida albicans, an opportunistic pathogen that kills immunocompromised hosts, have been identified using microarrays. The arrayed peptoid library was constructed using submonomers with moieties similar to ones found in small molecules known to bind RNA. Library members that passed quality control analysis were spotted onto a microarray and screened for binding to the C. albicans group I intron ribozyme. Each ligand binder identified from microarray-based screening inhibited self-splicing in the presence of 1 mM nucleotide concentration of bulk yeast tRNA with IC(50)'s between 150 and 2200 microM. The binding signals and the corresponding IC(50)'s were used to identify features in the peptoids that predispose them for RNA binding. After st

SUBMITTER: Labuda LP 

PROVIDER: S-EPMC2777746 | biostudies-literature | 2009 Apr

REPOSITORIES: biostudies-literature