Computational prediction of splicing regulatory elements shared by Tetrapoda organisms.

ABSTRACT:

Background

Auxiliary splicing sequences play an important role in ensuring accurate and efficient splicing by promoting or repressing recognition of authentic splice sites. These cis-acting motifs have been termed splicing enhancers and silencers and are located both in introns and exons. They co-evolved into an intricate splicing code together with additional functional constraints, such as tissue-specific and alternative splicing patterns. We used orthologous exons extracted from the University of California Santa Cruz multiple genome alignments of human and 22 Tetrapoda organisms to predict candidate enhancers and silencers that have reproducible and statistically significant bias towards annotated exonic boundaries.

Results

A total of 2,546 Tetrapoda enhancers and silenc

SUBMITTER: Churbanov A 

PROVIDER: S-EPMC2777938 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature