The mRNA distribution of C7orf24, a gamma-glutamyl cyclotransferase, in rat tissues.

ABSTRACT: The putative protein C7orf24 is encoded by Homo sapiens chromosome 7 open reading frame 24. C7orf24 was first identified as a 21-kDa cytochrome c-releasing factor detected in the cytosolic fraction of human leukemia U937 cells after treatment with geranylgeraniol. C7orf24 protein was recently identified as a gamma-glutamyl cyclotransferase, an enzyme in the gamma-glutamyl cycle. However, the exact localization of C7orf24 mRNA in normal tissues remains unknown. The present study examined the distribution pattern of C7orf24 mRNA in rat tissues using reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization histochemistry. The RT-PCR experiments demonstrated that C7orf24 and a variant C7orf24 mRNA were expressed in various tissues. Quantitative RT-PCR analysis reveale

SUBMITTER: Oda K 

PROVIDER: S-EPMC2778085 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature