Allergic sensitization through the airway primes Th17-dependent neutrophilia and airway hyperresponsiveness.

ABSTRACT:

Rationale

In humans, immune responses to inhaled aeroallergens develop in the lung and draining lymph nodes. Many animal models of asthma bypass this route and instead use intraperitoneal injections of allergen using aluminum hydroxide as an adjuvant.

Objectives

We investigated whether allergic sensitization through the airway elicits immune responses qualitatively different than those arising in the peritoneum.

Methods

Mice were sensitized to allergen through the airway using low-dose LPS as an adjuvant, or through the peritoneum using aluminum hydroxide as an adjuvant. After a single allergen challenge, ELISA and flow cytometry were used to measure cytokines and leukocyte subsets. Invasive measurements of airway resistance were used to measure allergen-induced airwa

SUBMITTER: Wilson RH 

PROVIDER: S-EPMC2778149 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature