Proteasome beta subunit pharmacogenomics: gene resequencing and functional genomics.

ABSTRACT:

Purpose

The proteasome is a multisubunit cellular organelle that functions as a nonlysosomal threonine protease. Proteasomes play a critical role in the degradation of proteins, regulating a variety of cellular processes, and they are also the target for antineoplastic proteasome inhibitors. Genetic variation in proteasome subunits could influence both proteasome function and response to drug therapy.

Experimental design

We resequenced genes encoding the three active proteasome beta subunits using 240 DNA samples from four ethnic groups and the beta 5 subunit gene in 79 DNA samples from multiple myeloma patients who had been treated with the proteasome inhibitor bortezomib. Resequencing was followed by functional studies of polymorphisms identified in the coding region and 3

SUBMITTER: Wang L 

PROVIDER: S-EPMC2778274 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature