BcMF9, a novel polygalacturonase gene, is required for both Brassica campestris intine and exine formation.

ABSTRACT:

Background and aims

The polygalacturonase (PG) gene family has been found to be enriched in pollen of several species; however, little is currently known about the function of the PG gene in pollen development. To investigate the exact role that the PG gene has played in pollen development and about this family in general, one putative PG gene, Brassica campestris Male Fertility 9 (BcMF9), was isolated from Chinese cabbage (Brassica campestris ssp. chinensis, syn. B. rapa ssp. chinensis) and characterized.

Methods

RT-PCR, northern blotting and in situ hybridization were used to analyse the expression pattern of BcMF9, and antisense RNA technology was applied to study the function of this gene.

Key results

BcMF9 is expressed in particular in the tapetum and microspore

SUBMITTER: Huang L 

PROVIDER: S-EPMC2778392 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature