Mutant p53 mediates survival of breast cancer cells.

ABSTRACT:

Background

p53 is the most commonly mutated tumour-suppressor gene in human cancers. Unlike other tumour-suppressor genes, most p53 cancer mutations are missense mutations within the core domain, leading to the expression of a full-length mutant p53 protein. Accumulating evidence has indicated that p53 cancer mutants not only lose tumour suppression activity but also gain new oncogenic activities to promote tumourigenesis.

Methods

The endogenous mutant p53 function in human breast cancer cells was studied using RNA interference (RNAi). Gene knockdown was confirmed by quantitative PCR and western blotting. Apoptosis was evaluated by morphological changes of cells, their PARP cleavage and annexin V staining.

Results

We show that cancer-associated p53 missense mutants ar

SUBMITTER: Lim LY 

PROVIDER: S-EPMC2778523 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature