Gene silencing of HPV16 E6/E7 induced by promoter-targeting siRNA in SiHa cells.

ABSTRACT:

Background

Recently, transcriptional gene silencing induced by small interfering RNA (siRNA) was found in mammalian and human cells. However, previous studies focused on endogenous genes.

Methods

In this study, we designed siRNA targeting the promoter of human papillomavirus 16 E6/E7 and transfected it into the cervical cancer cell line, SiHa. E6 and E7 mRNA and protein expression were detected in cells treated by promoter-targeting siRNA. Futhermore, cellular growth, proliferation, apoptosis and senescence were detected. Thereafter, we investigated promoter DNA methylation and histone methylation status in cells treated with promoter-targeting siRNA.

Results

We found that E6/E7 mRNA and protein were simultaneously reduced, cell growth and proliferation were inhibited

SUBMITTER: Hong D 

PROVIDER: S-EPMC2778536 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature