Gene expression profiling associated with the progression to poorly differentiated thyroid carcinomas.

ABSTRACT:

Background

Poorly differentiated thyroid carcinomas (PDTC) represent a heterogeneous, aggressive entity, presenting features that suggest a progression from well-differentiated carcinomas. To elucidate the mechanisms underlying such progression and identify novel therapeutic targets, we assessed the genome-wide expression in normal and tumour thyroid tissues.

Methods

Microarray analyses of 24 thyroid carcinomas - 7 classic papillary, 8 follicular variants of papillary (fvPTC), 4 follicular (FTC) and 5 PDTC - were performed and correlated with RAS, BRAF, RET/PTC and PAX8-PPARG alterations. Selected genes were validated by quantitative RT-PCR in an independent set of 28 thyroid tumours.

Results

Unsupervised analyses showed that gene expression similarity was higher betw

SUBMITTER: Pita JM 

PROVIDER: S-EPMC2778548 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature