Global effects of DNA replication and DNA replication origin activity on eukaryotic gene expression.

ABSTRACT: This report provides a global view of how gene expression is affected by DNA replication. We analyzed synchronized cultures of Saccharomyces cerevisiae under conditions that prevent DNA replication initiation without delaying cell cycle progression. We use a higher-order singular value decomposition to integrate the global mRNA expression measured in the multiple time courses, detect and remove experimental artifacts and identify significant combinations of patterns of expression variation across the genes, time points and conditions. We find that, first, approximately 88% of the global mRNA expression is independent of DNA replication. Second, the requirement of DNA replication for efficient histone gene expression is independent of conditions that elicit DNA damage checkpoint responses.

SUBMITTER: Omberg L 

PROVIDER: S-EPMC2779084 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature