Decreased growth factor expression through RNA interference inhibits development of mouse preimplantation embryos.

ABSTRACT: Mitogenic growth factors play an important role in cellular development and differentiation. The purpose of this study was to assess the extent to which epidermal growth factor (EGF) and transforming growth factor alpha (TGFalpha) and their cognate receptor (EGFR) are crucial for normal preimplantation embryo development. We used RNA interference to decrease expression of growth factors in preimplantation mouse embryos. We microinjected 1-cell mouse embryos individually with short interfering RNA (siRNA) specific to EGF, TGFalpha, and EGFR and then analyzed temporal and spatial gene expression patterns at different stages of development before implantation. Transfection with siRNA significantly reduced growth factor expression in 1-cell, 2-cell, morula, early-blastocyst, and late-blastocys

SUBMITTER: Dadi TD 

PROVIDER: S-EPMC2779207 | biostudies-literature | 2009 Aug

REPOSITORIES: biostudies-literature