Endogenous control genes in complex vascular tissue samples.

ABSTRACT:

Background

Gene expression microarrays and real-time PCR are common methods used to measure mRNA levels. Each method has a fundamentally different approach of normalization between samples. Relative quantification of gene expression using real-time PCR is often done using the 2(/\)(-DeltaDeltaCt) method, in which the normalization is performed using one or more endogenous control genes. The choice of endogenous control gene is often arbitrary or bound by tradition. We here present an analysis of the differences in expression results obtained with microarray and real-time PCR, dependent on different choices of endogenous control genes.

Results

In complex tissue, microarray data and real-time PCR data show the best correlation when endogenous control genes are omitted and the

SUBMITTER: Folkersen L 

PROVIDER: S-EPMC2779820 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature