New methods to analyse microarray data that partially lack a reference signal.

ABSTRACT:

Background

Microarray-based Comparative Genomic Hybridisation (CGH) has been used to assess genetic variability between bacterial strains. Crucial for interpretation of microarray data is the availability of a reference to compare signal intensities to reliably determine presence or divergence each DNA fragment. However, the production of a good reference becomes unfeasible when microarrays are based on pan-genomes.When only a single strain is used as a reference for a multistrain array, the accessory gene pool will be partially represented by reference DNA, although these genes represent the genomic repertoire that can explain differences in virulence, pathogenicity or transmissibility between strains. The lack of a reference makes interpretation of the data for these genes diffic

SUBMITTER: Carpaij N 

PROVIDER: S-EPMC2779823 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature