Microfluidic device for single-molecule experiments with enhanced photostability.

ABSTRACT: A microfluidic device made of polydimethylsiloxane (PDMS) addresses key limitations in single-molecule fluorescence experiments by providing high dye photostability and low sample sticking. Photobleaching is dramatically reduced by deoxygenation via gas diffusion through porous channel walls. Rapid buffer exchange in a laminar sheath flow followed by optical interrogation minimizes surface-sample contacts and allows the in situ addition and combination of other reagents.

SUBMITTER: Lemke EA 

PROVIDER: S-EPMC2779842 | biostudies-literature | 2009 Sep

REPOSITORIES: biostudies-literature