Detection of Helicobacter hepaticus in human bile samples of patients with biliary disease.

ABSTRACT:

Background

Since the discovery of Helicobacter pylori, various enterohepatic Helicobacter spices have been detected in the guts of humans and animals. Some enterohepatic Helicobacters have been associated with inflammatory bowel disease or liver disease in mice. However the association of these bacteria with human diseases remains unknown.

Materials and methods

We collected 126 bile samples from patients with cholelithiasis, cholecystitis, gallbladder polyp, and other nonbiliary diseases. Samples were screened for the presence of enterohepatic Helicobacter spp. using cultures, nested PCR, or in situ hybridization. We tested for antibodies to H. pylori and H. hepaticus by Western blot analysis.

Results

Attempts at cultivation were unsuccessful. However, H. hepaticus wa

SUBMITTER: Hamada T 

PROVIDER: S-EPMC2779990 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature