The C-terminal sequence of RhoB directs protein degradation through an endo-lysosomal pathway.

ABSTRACT:

Background

Protein degradation is essential for cell homeostasis. Targeting of proteins for degradation is often achieved by specific protein sequences or posttranslational modifications such as ubiquitination.

Methodology/principal findings

By using biochemical and genetic tools we have monitored the localization and degradation of endogenous and chimeric proteins in live primary cells by confocal microscopy and ultra-structural analysis. Here we identify an eight amino acid sequence from the C-terminus of the short-lived GTPase RhoB that directs the rapid degradation of both RhoB and chimeric proteins bearing this sequence through a lysosomal pathway. Elucidation of the RhoB degradation pathway unveils a mechanism dependent on protein isoprenylation and palmitoylation that

SUBMITTER: Perez-Sala D 

PROVIDER: S-EPMC2780327 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature