Enrichment and proteomic analysis of plasma membrane from rat dorsal root ganglions.

ABSTRACT:

Background

Dorsal root ganglion (DRG) neurons are primary sensory neurons that conduct neuronal impulses related to pain, touch and temperature senses. Plasma membrane (PM) of DRG cells plays important roles in their functions. PM proteins are main performers of the functions. However, mainly due to the very low amount of DRG that leads to the difficulties in PM sample collection, few proteomic analyses on the PM have been reported and it is a subject that demands further investigation.

Results

By using aqueous polymer two-phase partition in combination with high salt and high pH washing, PMs were efficiently enriched, demonstrated by western blot analysis. A total of 954 non-redundant proteins were identified from the plasma membrane-enriched preparation with CapLC-MS/MS an

SUBMITTER: Xiong X 

PROVIDER: S-EPMC2780401 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature