Comparison of Affymetrix Gene Array with the Exon Array shows potential application for detection of transcript isoform variation.

ABSTRACT:

Background

The emergence of isoform-sensitive microarrays has helped fuel in-depth studies of the human transcriptome. The Affymetrix GeneChip Human Exon 1.0 ST Array (Exon Array) has been previously shown to be effective in profiling gene expression at the isoform level. More recently, the Affymetrix GeneChip Human Gene 1.0 ST Array (Gene Array) has been released for measuring gene expression and interestingly contains a large subset of probes from the Exon Array. Here, we explore the potential of using Gene Array probes to assess expression variation at the sub-transcript level. Utilizing datasets of the high quality Microarray Quality Control (MAQC) RNA samples previously assayed on the Exon Array and Gene Array, we compare the expression measurements of the two platforms to det

SUBMITTER: Ha KCh 

PROVIDER: S-EPMC2780461 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature