Urocortin and corticotropin-releasing factor receptor 2 in human renal cell carcinoma: disruption of an endogenous inhibitor of angiogenesis and proliferation.

ABSTRACT:

Purpose

Urocortin (Ucn) exerts its actions through activation of two corticotropin-releasing factor receptors (CRFRs), CRFR1 and CRFR2. Involvement of Ucn/CRFR2 system in pathophysiological conditions such as the regulation of angiogenesis and inhibition of proliferation has been already reported. Suppression of neovascularization through reduction of vascular endothelial growth factor and inhibition of tumor cell cycling is modulated mainly through activation of CRFR2. To find out a possible involvement of Ucn/CRFR2 in kidney tumor, we examined the expression of Ucn and CRFR2 in normal and tumoral kidney specimens.

Methods

We applied reverse transcriptase PCR (n=14), immunofluorescence (IF) on tissue microarrays (n=25) and confocal microscopy to examine the mRNA expression

SUBMITTER: Tezval H 

PROVIDER: S-EPMC2780655 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature