Fine-scale variation and genetic determinants of alternative splicing across individuals.

ABSTRACT: Recently, thanks to the increasing throughput of new technologies, we have begun to explore the full extent of alternative pre-mRNA splicing (AS) in the human transcriptome. This is unveiling a vast layer of complexity in isoform-level expression differences between individuals. We used previously published splicing sensitive microarray data from lymphoblastoid cell lines to conduct an in-depth analysis on splicing efficiency of known and predicted exons. By combining publicly available AS annotation with a novel algorithm designed to search for AS, we show that many real AS events can be detected within the usually unexploited, speculative majority of the array and at significance levels much below standard multiple-testing thresholds, demonstrating that the extent of cis-regulated differ

SUBMITTER: Coulombe-Huntington J 

PROVIDER: S-EPMC2780703 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature