Virus-induced autoimmune diabetes in the LEW.1WR1 rat requires Iddm14 and a genetic locus proximal to the major histocompatibility complex.

ABSTRACT:

Objective

To identify genes that confer susceptibility to autoimmune diabetes following viral infection in the LEW.1WR1 rat.

Research design and methods

About 2% of LEW.1WR1 rats develop spontaneous autoimmune diabetes. Immunological perturbants including viral infection increase both the frequency and tempo of diabetes onset. To identify diabetes susceptibility genes (LEW.1WR1 x WF), F2 rats were infected with Kilham rat virus following brief pretreatment with polyinosinic:polycytidylic acid. This treatment induces diabetes in 100% of parental LEW.1WR1 rats and 0% of parental WF rats. Linkage to diabetes was analyzed by genome-wide scanning.

Results

Among 182 F2 rats, 57 (31%) developed autoimmune diabetes after a mean latency of 16 days. All diabetic animals and app

SUBMITTER: Blankenhorn EP 

PROVIDER: S-EPMC2780864 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature