Functional role of neuroendocrine-specific protein-like 1 in membrane translocation of GLUT4.

ABSTRACT:

Objective

In skeletal muscles, dantrolene inhibits the exercise-induced membrane translocation of GLUT4. It has been postulated that the inhibitory action of dantrolene on Ca(2+) release from the sarcoplasmic reticulum (SR) causes inhibition of exercise-induced glucose uptake; however, the precise mechanism has not been adequately studied.

Research design and methods

We discovered that dantrolene can bind to skeletal-type neuroendocrine-specific protein-like 1 (sk-NSPl1) with photoreactive dantrolene derivatives. In sk-NSPl1-deficient muscles, we examined the change in glucose uptake and the membrane translocation of GLUT4. In addition, we examined the change in blood glucose and also measured the glycogen level in both isolated and in situ skeletal muscles after electrical

SUBMITTER: Ikemoto T 

PROVIDER: S-EPMC2780876 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature