Insulin resistance and altered systemic glucose metabolism in mice lacking Nur77.

ABSTRACT:

Objective

Nur77 is an orphan nuclear receptor with pleotropic functions. Previous studies have identified Nur77 as a transcriptional regulator of glucose utilization genes in skeletal muscle and gluconeogenesis in liver. However, the net functional impact of these pathways is unknown. To examine the consequence of Nur77 signaling for glucose metabolism in vivo, we challenged Nur77 null mice with high-fat feeding.

Research design and methods

Wild-type and Nur77 null mice were fed a high-fat diet (60% calories from fat) for 3 months. We determined glucose tolerance, tissue-specific insulin sensitivity, oxygen consumption, muscle and liver lipid content, muscle insulin signaling, and expression of glucose and lipid metabolism genes.

Results

Mice with genetic deletion of

SUBMITTER: Chao LC 

PROVIDER: S-EPMC2780886 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature