A quantification of pathway components supports a novel model of Hedgehog signal transduction.

ABSTRACT: The secreted protein Hedgehog (Hh) plays a critical instructional role during metazoan development. In Drosophila, Hh signaling is interpreted by a set of conserved, downstream effectors that differentially localize and interact to regulate the stability and activity of the transcription factor Cubitus interruptus. Two essential models that integrate genetic, cell biological, and biochemical information have been proposed to explain how these signaling components relate to one another within the cellular context. As the molar ratios of the signaling effectors required in each of these models are quite different, quantitating the cellular ratio of pathway components could distinguish these two models. Here, we address this important question using a set of purified protein standards to perf

SUBMITTER: Farzan SF 

PROVIDER: S-EPMC2781433 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature