The transcriptional repressor CcpN from Bacillus subtilis uses different repression mechanisms at different promoters.

ABSTRACT: CcpN, a transcriptional repressor from Bacillus subtilis that is responsible for the carbon catabolite repression of three genes, has been characterized in detail in the past 4 years. However, nothing is known about the actual repression mechanism as yet. Here, we present a detailed study on how CcpN exerts its repression effect at its three known target promoters of the genes sr1, pckA, and gapB. Using gel shift assays under non-repressive and repressive conditions, we showed that CcpN and RNA polymerase can bind simultaneously and that CcpN does not prevent RNA polymerase (RNAP) binding to the promoter. Furthermore, we investigated the effect of CcpN on open complex formation and demonstrate that CcpN also does not act at this step of transcription initiation at the sr1 and pckA and pres

SUBMITTER: Licht A 

PROVIDER: S-EPMC2781557 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature