Proteomics reveals a physical and functional link between hepatocyte nuclear factor 4alpha and transcription factor IID.

ABSTRACT: Proteomic analyses have contributed substantially to our understanding of diverse cellular processes. Improvements in the sensitivity of mass spectrometry approaches are enabling more in-depth analyses of protein-protein networks and, in some cases, are providing surprising new insights into well established, longstanding problems. Here, we describe such a proteomic analysis that exploits MudPIT mass spectrometry and has led to the discovery of a physical and functional link between the orphan nuclear receptor hepatocyte nuclear factor 4alpha (HNF4alpha) and transcription factor IID (TFIID). A systematic characterization of the HNF4alpha-TFIID link revealed that the HNF4alpha DNA-binding domain binds directly to the TATA box-binding protein (TBP) and, through this interaction, can target T

SUBMITTER: Takahashi H 

PROVIDER: S-EPMC2781655 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature