Interaction of HP1 and Brg1/Brm with the globular domain of histone H3 is required for HP1-mediated repression.

ABSTRACT: The heterochromatin-enriched HP1 proteins play a critical role in regulation of transcription. These proteins contain two related domains known as the chromo- and the chromoshadow-domain. The chromo-domain binds histone H3 tails methylated on lysine 9. However, in vivo and in vitro experiments have shown that the affinity of HP1 proteins to native methylated chromatin is relatively poor and that the opening of chromatin occurring during DNA replication facilitates their binding to nucleosomes. These observations prompted us to investigate whether HP1 proteins have additional histone binding activities, envisioning also affinity for regions potentially occluded by the nucleosome structure. We find that the chromoshadow-domain interacts with histone H3 in a region located partially inside th

SUBMITTER: Lavigne M 

PROVIDER: S-EPMC2782133 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature