Gene and protein responses of human monocytes to extracellular cysteine redox potential.

ABSTRACT: The redox potential of the major thiol/disulfide couple, cysteine (Cys) and its disulfide cystine (CySS), in plasma (E(h)Cys) is oxidized in association with oxidative stress, and oxidized E(h)Cys is associated with cardiovascular disease risk. In vitro exposure of monocytes to oxidized E(h)Cys increases expression of the proinflammatory cytokine, interleukin-1beta (IL-1beta), suggesting that E(h)Cys could be a mechanistic link between oxidative stress and chronic inflammation. Because cell membranes contain multiple Cys-rich proteins, which could be sensitive to E(h)Cys, we sought to determine whether E(h)Cys specifically affects proinflammatory signaling or has other effects on monocytes. We used microarray analysis and mass spectrometry-based proteomics to evaluate global changes in pro

SUBMITTER: Go YM 

PROVIDER: S-EPMC2782258 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature