Affinity maturation generates greatly improved xyloglucan-specific carbohydrate binding modules.

ABSTRACT:

Background

Molecular evolution of carbohydrate binding modules (CBM) is a new approach for the generation of glycan-specific molecular probes. To date, the possibility of performing affinity maturation on CBM has not been investigated. In this study we show that binding characteristics such as affinity can be improved for CBM generated from the CBM4-2 scaffold by using random mutagenesis in combination with phage display technology.

Results

Two modified proteins with greatly improved affinity for xyloglucan, a key polysaccharide abundant in the plant kingdom crucial for providing plant support, were generated. Both improved modules differ from other existing xyloglucan probes by binding to galactose-decorated subunits of xyloglucan. The usefulness of the evolved binders was

SUBMITTER: von Schantz L 

PROVIDER: S-EPMC2783032 | biostudies-literature | 2009 Oct

REPOSITORIES: biostudies-literature