Development of a novel monoclonal antibody with reactivity to a wide range of Venezuelan equine encephalitis virus strains.

ABSTRACT:

Background

There is currently a requirement for antiviral therapies capable of protecting against infection with Venezuelan equine encephalitis virus (VEEV), as a licensed vaccine is not available for general human use. Monoclonal antibodies are increasingly being developed as therapeutics and are potential treatments for VEEV as they have been shown to be protective in the mouse model of disease. However, to be truly effective, the antibody should recognise multiple strains of VEEV and broadly reactive monoclonal antibodies are rarely and only coincidentally isolated using classical hybridoma technology.

Results

In this work, methods were developed to reliably derive broadly reactive murine antibodies. A phage library was created that expressed single chain variable fragmen

SUBMITTER: O'Brien LM 

PROVIDER: S-EPMC2783036 | biostudies-literature | 2009 Nov

REPOSITORIES: biostudies-literature