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TU-tagging: cell type-specific RNA isolation from intact complex tissues.

ABSTRACT: We found that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil delivery can be used to label and purify cell type-specific RNA from intact complex tissues in Drosophila melanogaster. This method is useful for isolating RNA from cell types that are difficult to isolate by dissection or dissociation methods and should work in many organisms, including mammals and other vertebrates.

SUBMITTER: Miller MR 

PROVIDER: S-EPMC2783170 | biostudies-literature | 2009 Jun

REPOSITORIES: biostudies-literature

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TU-tagging: cell type-specific RNA isolation from intact complex tissues.

Miller Michael R MR   Robinson Kristin J KJ   Cleary Michael D MD   Doe Chris Q CQ  

Nature methods 20090510 6

We found that the combination of spatially restricted uracil phosphoribosyltransferase (UPRT) expression with 4-thiouracil delivery can be used to label and purify cell type-specific RNA from intact complex tissues in Drosophila melanogaster. This method is useful for isolating RNA from cell types that are difficult to isolate by dissection or dissociation methods and should work in many organisms, including mammals and other vertebrates. ...[more]

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