Eotaxin increases monolayer permeability of human coronary artery endothelial cells.

ABSTRACT:

Objective

The objective of this study was to determine the effects and molecular mechanisms of eotaxin, a newly discovered chemokine (CCL11), on endothelial permeability in the human coronary artery endothelial cells (HCAECs).

Methods and results

Cells were treated with eotaxin, and the monolayer permeability was studied by using a costar transwell system with a Texas Red-labeled dextran tracer. Eotaxin significantly increased monolayer permeability in a concentration-dependent manner. In addition, eotaxin treatment significantly decreased the mRNA and protein levels of endothelial junction molecules including zonula occludens-1 (ZO-1), occludin, and claudin-1 in a concentration-dependent manner as determined by real-time RT-PCR and Western blot analysis, respectively. Incre

SUBMITTER: Jamaluddin MS 

PROVIDER: S-EPMC2784176 | biostudies-literature | 2009 Dec

REPOSITORIES: biostudies-literature